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Table 2 Summary of results obtained from parameter estimation analyses

From: Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides

Parameter

PE18

PE19

PE20

Value

Value

Value

CdP reaction

 KiGlc1P, [mM]

0.32

0.32

0.32

 KGlc1P, [mM]

0.72

0.72

0.72

 KG2, [mM]

6.22

6.23

6.14

 KG3, [mM]

24.5

24.5

24.5

 KG4 (= KG5KG11), [mM]

98.6

99.1

58.5

 Keq

1.03

1.03

1.04

 k2, [−]

1.94

1.94

1.98

 k3, [−]

4.57

4.59

2.80

 k4, [−]

4.31

4.33

2.66

 k5 (= k6k10), [−]

1.56

1.56

1.00

Precipitation

 [G5]SL, [mM]

14.73

14.35

14.59

 [G6]SL, [mM]

2.68

2.68

2.68

 [G7]SL, [mM]

0.896

0.896

0.896

 [G8]SL, [mM]

0.1620

0.1674

0.1672

 [G9]SL, [mM]

0.0308

0.0313

0.0313

 [G10]SL, [mM]

0.00331

0.00333

0.00585

 [G11]SL, [mM]

0.001084

0.001087

0.000706

 [G12]SL, [mM]

0.000114

0.000196

0.000114