Fig. 3

Initial activity screens of FgrAAO and FoxAAO. Measurements were performed in triplicate at 25 °C in 50 mM sodium phosphate buffer, pH 7.5, using the HRP/ABTS assay. Activities were monitored using 300 mM for carbohydrates, polyols, diols and primary alcohols, 2.5 mg mL⁻¹ for polysaccharides, 30 mM for benzyl alcohol and galactitol, 5 mM for methyl glyoxal, aryl alcohols and furans and 10 mM for secondary alcohols. Reactions were started with the addition of 6 pmol–1 nmole of purified enzyme (Additional file 1: Table S2)