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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Modular biosynthesis of plant hemicellulose and its impact on yeast cells

Fig. 1

Modular engineering of hemicellulose synthesis. a Model of the potential cell phenotypes and future applications, such as biological capsules for therapeutic proteins, of yeast engineered to produce plant matrix polysaccharides. b Topology of AtCSLA2 and AkCSLA3 enzymes visualized with TOPCONS 2.0. Transmembrane domains (pink boxes), and regions inside (red) and outside (blue) the membrane. The GT2 domain denotes the conserved GT2 domain (Pfam PF13641). Three regions (dashed lines) with 100% amino acid identity were selected as borders for CSLA domain swapping. c Workflow for the assembly of chimeric DNA using the GoldenPICS cloning system. CSL domains are fused seamlessly in a level 1 vector, and then assembled with a yeast promoter and a transcriptional terminator into a level 2 vector for stable yeast transformation. Selected yeast colonies were induced to express the CSL enzymes using methanol and were subjected to cell wall analyses. d Matrix and labeling of AtCSLA2/AkCSLA3 single-domain swaps assembled in this study

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