Fig. 4
From: Modular biosynthesis of plant hemicellulose and its impact on yeast cells
Structure of (gluco)mannans produced by the top chimeric CSLAs. a Carbohydrates released by endo-β-1,4-mannanase digestion of alkaline-insoluble polymers. Solubilized carbohydrates were subjected to TFA-hydrolysis prior to HPEAC-PAD analysis. Data show the mean + SD of three biological replicates, re-grown based on the most promising Pichia strains from Figs. 2, 3. b Relative abundance of glucomannan glycosidic linkages in enriched mannan (EM) samples after Zymolyase treatment. Data show the mean + SD of three measurements for each sample. c Relative peak area of mannan (Man1-5) and glucomannan (GlcMan) oligosaccharides released from Pichia EM by mannanase relative to ivory nut mannan (INM) and konjac glucomannan (KGM) standards. Data show the mean + SD of two measurements. In a–c, significant differences between samples are marked by different letters (one-way ANOVA with Tukey test, P < 0.05). d HPAEC-PAD oligosaccharide profiles of mannanase-treated samples quantified in c. GlcMan diagnostic peaks, based on the controls in Additional file 1: Figures S4, are marked by asterisks