Fig. 7
From: Modular biosynthesis of plant hemicellulose and its impact on yeast cells
CSL enzyme features and further enhancement of hemicellulose synthesis. a Multiple sequence alignment, showing the selected borders for the chimeric proteins (dashed lines) and additional conserved motifs among active CSLAs (green lines). Arrowheads mark residues involved in glucan coordination by the structural studies of the PttCesA8 enzyme in Pichia. The shading is proportional to the amino acid similarity. TM, transmembrane domains. b Swapped CSLA domains are particularly boosted by catalytic domain by AtMSR1 to surpass parental yields, based on absolute monosaccharide composition. c Monosaccharide composition of carbohydrates released from AKI by β-mannanase digestion. In b, c dots show two technical replicates (each measured in duplicate) and significant differences between samples are marked by different letters (one-way ANOVA with Tukey test, P < 0.05)