Fig. 2

Isolation of RG-II using size-exclusion chromatography (SEC). a EPG-solubilized material (200–300 mg) from celery AIR in 50 mM NaOAc pH 5 was fractionated using a preparative Sephadex G-75 SEC column (1 m × 4 cm). Fractions were assayed colorimetrically for uronic acids (A₅₃₀). b SEC of the EPG-treated oxalate-soluble fraction from W. repanda fractionated on a Superdex 75 Increase column monitored using refractive index (RI) detection. c The ethanol precipitated material from red wine (~ 500 mg) separated using a preparative Sephadex G-75 SEC column (1 m × 4 cm) and assayed colorimetrically for uronic acids (A₅₃₀)