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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II

Fig. 5

Locating the O-acetyl groups of side chain B and the methyl-ethers of side chain A using ESI–MS. a The ESI mass spectrum of the oligosaccharides generated by treating wine RG-II with 0.1 M TFA for 16 h at 40 °C (side chain B-enriched). ∆146 corresponds to a Rha residue, ∆132 corresponds to an Ara residue, and ∆42 corresponds to an OAc group. b The ms2 spectra of the B side chain nonasaccharide (m/z 1357) and its fragmentation pattern. c The ms2 spectra of the B side chain mono-O-acetylated nonasaccharide (m/z 1399) and its fragmentation pattern. d The ESI mass spectrum of the oligosaccharides generated by treating wine RG-II with 0.1 M TFA for 1 h at 80 °C (side chain A-enriched). ∆14 corresponds to a methyl group. e The ms2 spectra of the A side chain octasaccharide (m/z 1301) and its fragmentation pattern. f The ms2 spectra of the A side chain mono-methylated octasaccharide (m/z 1315) and its fragmentation pattern. Refer to Fig. 1c for the relevant symbol nomenclature for glycans

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