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Table 2 Partial glycosyl residue compositions of purified wine and celery RG-II

From: Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II

Glycose

Wine RG-II (Sephadex G-75 fraction)a

Celery RG-II (Q-Sepharose 1.5 M imidazole fraction)b

Mol%

MeFuc

2.9 ± 0.1

3.9 ± 0.4

Rha

16.3 ± 0.7

14.9 ± 0.9

Fuc

1.8 ± 0.0

2.9 ± 0.1

MeXyl

2.9 ± 0.2

4.0 ± 0.4

Ara

17.6 ± 1.0

14.1 ± 1.5

Api

3.6 ± 0.3

3.9 ± 0.8

AceA

1.2 ± 0.1

1.3 ± 0.3

Gal

17.9 ± 1.2

10.7 ± 1.0

Glc

1.4 ± 0.1

0.2 ± 0.0

GalA

29.2 ± 1.2

39.9 ± 3.8

GlcA

5.2 ± 2.6

4.1 ± 1.1

  1. Quantification of neutral sugars and AceA was performed using alditol acetate derivatives. Quantification of uronic acids was performed using HPAEC-PAD. All values are expressed as a molar percentage and represent the average data from three replicates. See Additional file 1: Figure S2 for representative spectra
  2. aThe RG-II fraction isolated by SEC of the material solubilized by EPG treatment of celery petiole AIR (see Fig. 2 in the main text)
  3. bThe celery RG-II obtained by SEC was purified by anion-exchange chromatography. A solution of the celery RG-II (400 mg) in 10 mM imidazole–HCl pH 7 was applied to a column (15 cm × 2 cm; 47.1 mL column volume) of fast flow DEAE-Sepharose (Cytiva, USA). The column was eluted stepwise with 10 mM imidazole–HCl pH 7 (3 column volumes), 100 mM imidazole–HCl pH 7 (3 column volumes), and then with 1.5 M imidazole–HCl, pH 7 (4 column volumes). RG-II (~ 95% dimer, 295 mg) eluted with 1.5 M imidazole–HCl. A galactose-rich material (73 mg) eluted with 10 mM imidazole–HCl