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Table 4 Kinetics parameters for adsorption of enzyme onto lignin films, as measured by SPR

From: Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lignin

ka

kd

Rmax

KD

(M−1S−1)

(S−1)

(RU)

(nM)

DAP-MWL

3.1 × 104

7.68 × 10–4

497

24.7

Dio-MWL

4.0 × 104

2.70 × 10–4

433

6.8

Eth-MWL

3.1 × 104

5.10 × 10–4

406

16.3

THF-MWL

3.3 × 104

4.28 × 10–4

411

13.2

Dio-SL

3.4 × 104

1.57 × 10–3

493

48.9

Eth-SL

2.7 × 104

1.46 × 10–3

506

52.6

THF-SL

5.5 × 104

2.18 × 10–3

486

39.4

  1. ka association rate constant
  2. kd dissociation rate constant
  3. Rmax maximum amount of cellulase bound to lignin film
  4. KD equilibrium dissociation constant
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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