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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: CAZyme prediction in ascomycetous yeast genomes guides discovery of novel xylanolytic species with diverse capacities for hemicellulose hydrolysis

Fig. 6

Characterization of non-sequenced xylanolytic yeasts. a Phylogenetic analysis of 19 Blastobotrys, Sugiyamaella and Scheffersomyces species as well as Schizosaccharomyces pombe serving as outgroup. The molecular phylogenetic analysis was based on ITS sequences using maximum-likelihood model from ClustalW alignment with 1000 bootstrap replicates. The numbers at each branch indicate bootstrap values and tree topology confidence. The tree is drawn to scale, with branch lengths measured in the number (0.2) of substitutions per site. Growth profiles of xylanolytic yeasts grown in Delft medium containing 10 g L−1 of b wheat arabinoxylan and c birchwood glucuronoxylan. Yeasts were grown for 48 h at 30 °C. GV = Green Value (corresponding to growth based on pixel counts, as determined by a GrowthProfiler instrument). d Secretome and e cell-associated volumetric xylanase activities on wheat arabinoxylan (grey) and birchwood glucuronoxylan (black) determined at 30 °C after growth in xylan-containing liquid medium for 72 h. Stars (*) symbolizes non-sequenced species

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