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Table 3 Applications of plasticity engineering for microbial production of monoterpenoids

From: Plasticity engineering of plant monoterpene synthases and application for microbial production of monoterpenoids

Applications

MTPSs/variants

Hosts

Substrates

Titer or major products

Ref.

Exploiting orthogonal pathway

MsLimS

E. coli

GPP

181.73 mg/L limonene

[74]

MsLimS

E. coli

NPP

694.61 mg/L limonene

ClLimS2

S. cerevisiae

GPP

141.6 mg/L limonene

[73]

ClLimS2

S. cerevisiae

NPP

917.7 mg/L limonene

ArLimS

Y. lipolytica

GPP

Limonene was undetected

[75]

ArLimS

Y. lipolytica

NPP

23.56 mg/L limonene

ClLimS

S. cerevisiae

GPP

27.97 mg/L limonene

[31]

ClLimSH570Y

S. cerevisiae

NPP

134.81 mg/L limonene

SpSabS

S. cerevisiae

GPP

17.67 mg/L sabinene

[31]

SpSabSH561F

S. cerevisiae

NPP

72.39 mg/L sabinene

Enhancing enzymatic activity

ObGerS

S. cerevisiae

GPP

8.4 mg/L geraniol

[77]

ObGerSF355Y/ ObGerSD507H

S. cerevisiae

GPP

 ~ 10.7 mg/L geraniol

CrGerS

S. cerevisiae

GPP

54.8 mg/L geraniol

[78]

CrGerSF418Q

S. cerevisiae

GPP

66.8 mg/L geraniol

MsLimS

E. coli

GPP

 ~ 542 mg/L limonene

[32]

MsLimSG566A−L571F

E. coli

GPP

 ~ 940 mg/L limonene

PtPinS

E. coli

GPP

80 mg/L pinene

[79]

PtPinS(PT1)Q457L

E. coli

GPP

150 mg/L pinene

PtPinS

Cyanobacteria

GPP

 ~ 40 μg/L pinene

[79]

PtPinS(PT1)Q457L

Cyanobacteria

GPP

80 μg/L pinene

Altering product spectrum

SeCamS

S. cerevisiae

GPP

 ~ 50% camphene

[31]

SeCamsH583V/ SeCamsH583F

S. cerevisiae

NPP

Limonene predominantly

ClLimS

S. cerevisiae

GPP

99% C10 monoterpenoids

[61]

ClLimSH570V/

ClLimSH570L/

ClLimSH570I

S. cerevisiae

2meGPP

70%–95% C11 terpenoids (69%–78% 2-methyllimonene of C11 products)

PtPinS(PT30)

S. cerevisiae

GPP

 ~ 75% C10 monoterpenoids

[61]

PtPinS(PT30)F607L

S. cerevisiae

2meGPP

 > 80% C11 terpenoids (56% 2-methyllinalool of C11 products)

PtPinS(PT30)F607I

S. cerevisiae

2meGPP

 > 80% C11 terpenoids (75% 2-methylenebornane of C11 products)