Applications | MTPSs/variants | Hosts | Substrates | Titer or major products | Ref. |
---|---|---|---|---|---|
Exploiting orthogonal pathway | MsLimS | E. coli | GPP | 181.73Â mg/L limonene | [74] |
MsLimS | E. coli | NPP | 694.61Â mg/L limonene | ||
ClLimS2 | S. cerevisiae | GPP | 141.6Â mg/L limonene | [73] | |
ClLimS2 | S. cerevisiae | NPP | 917.7Â mg/L limonene | ||
ArLimS | Y. lipolytica | GPP | Limonene was undetected | [75] | |
ArLimS | Y. lipolytica | NPP | 23.56Â mg/L limonene | ||
ClLimS | S. cerevisiae | GPP | 27.97Â mg/L limonene | [31] | |
ClLimSH570Y | S. cerevisiae | NPP | 134.81Â mg/L limonene | ||
SpSabS | S. cerevisiae | GPP | 17.67Â mg/L sabinene | [31] | |
SpSabSH561F | S. cerevisiae | NPP | 72.39Â mg/L sabinene | ||
Enhancing enzymatic activity | ObGerS | S. cerevisiae | GPP | 8.4Â mg/L geraniol | [77] |
ObGerSF355Y/ ObGerSD507H | S. cerevisiae | GPP |  ~ 10.7 mg/L geraniol | ||
CrGerS | S. cerevisiae | GPP | 54.8Â mg/L geraniol | [78] | |
CrGerSF418Q | S. cerevisiae | GPP | 66.8Â mg/L geraniol | ||
MsLimS | E. coli | GPP |  ~ 542 mg/L limonene | [32] | |
MsLimSG566A−L571F | E. coli | GPP |  ~ 940 mg/L limonene | ||
PtPinS | E. coli | GPP | 80Â mg/L pinene | [79] | |
PtPinS(PT1)Q457L | E. coli | GPP | 150Â mg/L pinene | ||
PtPinS | Cyanobacteria | GPP |  ~ 40 μg/L pinene | [79] | |
PtPinS(PT1)Q457L | Cyanobacteria | GPP | 80 μg/L pinene | ||
Altering product spectrum | SeCamS | S. cerevisiae | GPP |  ~ 50% camphene | [31] |
SeCamsH583V/ SeCamsH583F | S. cerevisiae | NPP | Limonene predominantly | ||
ClLimS | S. cerevisiae | GPP | 99% C10 monoterpenoids | [61] | |
ClLimSH570V/ ClLimSH570L/ ClLimSH570I | S. cerevisiae | 2meGPP | 70%–95% C11 terpenoids (69%–78% 2-methyllimonene of C11 products) | ||
PtPinS(PT30) | S. cerevisiae | GPP |  ~ 75% C10 monoterpenoids | [61] | |
PtPinS(PT30)F607L | S. cerevisiae | 2meGPP |  > 80% C11 terpenoids (56% 2-methyllinalool of C11 products) | ||
PtPinS(PT30)F607I | S. cerevisiae | 2meGPP |  > 80% C11 terpenoids (75% 2-methylenebornane of C11 products) |