Fig. 2
Fluorescence strength of the promoters. a Characterization of the native promoters PLEU, PEXP and PTEF. b Characterization of different core promoters, and the relationship between T content upstream of TSS and relative mean fluorescence. Bars represent relative mean fluorescence and lines represent percentage of T content upstream of TSS. c Characterization of different TATA boxes. d Relationship between the copy number of UAS and relative mean fluorescence. e Characterization of different UASs from S. cerevisiae and Y. lipolytica. At least three biological replicates were measured by flow cytometry, and a cell count of 10,000 was analyzed. The error bars represent standard deviation