Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study

Table 1 List of promoters used in this study

Promoters	UAS type	TATA box	Core promoter	Strength	References
LEU				+	[40]
EXP				+	[31]
TEF				+ +	[31]
LEUm		LEU	LEU	+	[12]
UASTEFSC4-LEUm	UASTEFSC4	LEU	LEU	+	This study
UASCLBSC4-LEUm	UASCLBSC4	LEU	LEU	+	This study
UASCITSC4-LEUm	UASCITSC4	LEU	LEU	+ +	This study
UAS1B4-EXPm	UAS1B4	EXP	EXP	+ +	This study
UAS1B4-POX2m	UAS1B4	POX2	POX2	+ +	This study
UAS1B4-TATAPAT1-LEU	UAS1B4	PAT1	LEU	+ +	This study
UAS1B4-PAT1m	UAS1B4	PAT1	PAT1	+ + +	This study
UASTEFYL4-LEUm	UASTEFYL4	LEU	LEU	+ + +	This study
UAS1B4-TEF175	UAS1B4	TEF	TEF175	+ + +	This study
UAS1B4-TEF136	UAS1B4	TEF	TEF136	+ + +	This study
UAS1B4-TEF111	UAS1B4	TEF	TEF111	+ + +	This study
UAS1B4-LEUm	UAS1B4	LEU	LEU	+ + + +	[20]
UAS1B4-TATAPOX2-LEU	UAS1B4	POX2	LEU	+ + + +	This study
UAS1B4-TATAEXP-LEU	UAS1B4	EXP	LEU	+ + + +	This study
UAS1B6-LEUm	UAS1B6	LEU	LEU	+ + + + +	This study
UAS1B4-TATATEF-LEU	UAS1B4	TEF	LEU	+ + + + +	This study
UAS1B8-LEUm	UAS1B8	LEU	LEU	+ + + + +	[26]

+ Means the range of relative mean fluorescence is 0–10,000; + + means the range of relative mean fluorescence is 10,000–50,000; + + + means the range of relative mean fluorescence is 50,000–100,000; + + + + means the range of relative mean fluorescence is 100000–150,000; + + + + + means the range of relative mean fluorescence is > 150,000

ISSN: 2731-3654