Fig. 4

Overall structure of ODH and sequence alignment with homologous enzymes of known structure. a Sequence alignment of ODH, AfGDH and AnGOX based on structural superposition. Numbers refer to ODH sequence; conserved residues are colored in red. Secondary structure elements are assigned for ODH: β-strands are in orange and α-helices in light blue. b Ribbon representation of the whole three-dimensional structure of ligand-free ODH, with secondary structures assignment for β-sheets and α-helices (H). The FAD-binding domain is shown in yellow, the substrate-binding domain in light blue, the FAD cofactor in dark red sticks. Glycosylations are represented in sticks, with C atoms in yellow or light blue, O in red and N in blue