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Table 1 Apparent ODH kinetic constants for GLC and G3G

From: Crystal structure and functional characterization of an oligosaccharide dehydrogenase from Pycnoporus cinnabarinus provides insights into fungal breakdown of lignocellulose

  KM (mM) kcat (s−1) kcat/KM* (M−1 s−1) kcat/KM** (M−1 s−1)
GLC 755 ± 110 50 ± 3 67 ± 10 47 ± 1
G3G 77 ± 10 71 ± 4 917 ± 129 777 ± 21
  1. *kcat/KM calculated from the apparent KM and kcat estimated from fitting the Michaelis–Menten hyperbola to data points (Fig. 3)
  2. **kcat/KM calculated as the slope of the tangent of the Michaelis–Menten hyperbola in its linear region (insets in Fig. 3)