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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Stress tolerance enhancement via SPT15 base editing in Saccharomyces cerevisiae

Fig. 2

Computational scanning and experimental mutagenesis of SPT15 via Target-AID base editor. a Predicted effects of Spt15 amino acid residues. The predicted functions of amino acid residues were retrieved by artificially applying a point mutation of alanine at each position from the MutFunc database (http://www.mutfunc.com/), which has precomputed data for all possible mutations. Numbers of different variant consequences were counted and labeled on the pie chart. b The procedure of in situ mutagenizing Spt15 via base editing as well as stress tolerance genotyping and phenotyping of Spt15 mutant strains

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