Fig. 7
From: Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Overexpression of antioxidant genes to improve the heterologous protein expression ability of P. pastoris. A The antioxidant defence system of yeasts. O2·−: superoxide anion; SOD: superoxide dismutase; CAT: catalase; GPx: glutathione peroxidase; GLR1: glutathione reductase; Prx: peroxiredoxin/thioredoxin peroxidase; Trx: thioredoxin; TRR1: thioredoxin reductase; G6PD: glucose-6-phosphate dehydrogenase; 6PD: 6-phosphogluconate dehydrogenase; GSH: glutathione; γ-GC: gamma glutamylcysteine; l-glu: l-glutamic acid; l-cys: l-cysteine. B Relative transcription levels of antioxidant system-related genes. The expression ratio of a gene was analysed using the 2−ΔΔCt method; C the intracellular ROS level of overexpressed strains and their blank control during methanol-induced fermentation; D foldchange of lipase activity levels in overexpressed strains. *p < 0.05, **p < 0.01