Fig. 2

Biomass, lipid accumulation, and ROS levels of Schizochytrium limacinum SR21. A Biomass. B Percentage of the total fatty acid content to biomass. C Fatty acid composition of the total fatty acid at 5rd of fermentation. D Percentage of the primary fatty acids to total lipids after 5 days of fermentation. The different lowercase letters in each bar indicate a significant difference (p < 0.05) between different strains. E Spearman pairwise correlation globe among the different fatty acids. Each line represents a Spearman's correlation coefficient between two different fatty acids. Positive correlations are indicated in red, and the negative correlations are in green. F Reactive oxygen species (ROS) levels. Standard bars represent the standard deviation of triplicate samples. WT, ER⁺, and DH⁺ represent the wild-type strain, DH-overexpression strain, and ER-overexpression strain, respectively