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Table 1 Composition of CWR and percentage of solubilized or lost macromolecular fractions after the HWP

From: Evaluating polymer interplay after hot water pretreatment to investigate maize stem internode recalcitrance

  Raw maize stem 20-min HWP 40-min HWP
M7 M9 M7 M9 M7 M9
Pretreatment severity 3.66 3.66 3.96 3.96
Yield of HWP (g g−1 CWR) - 0.797 (± 0.022) 0.796 (± 0.034) 0.629 (± 0.027) 0.636 (± 0.033)
Hydrolysate pH 4.4 (± 0.06) 4.4 (± 0.08) 3.8 (± 0.01) 3.8 (± 0.06)
Composition
 Polysaccharides (g 100 g−1 CWR) 60.2 (± 0.7) 67.3 (± 1.5) 65.7 (± 1.9) 62.1 (± 4.4) 65.1 (± 2.6) 64.0 (± 0.3)
 Cellulose (g.100 g−1 of sugars) 67.7 (± 0.1) 62.1 (± 0.4) 58.4 (± 0.1) 62.4 (± 0.3) 76.7 (± 0.3) 69.2 (± 0.8)
 Hemicelluloses (g.100 g−1 of sugars) 32.3 (± 0.1) 37.9 (± 0.4) 41.6 (± 0.1) 37.6 (± 0.3) 23.3 (± 0.3) 30.8 (± 0.8)
 Klason lignin (g.100 g−1 CWR) 16.3 (± 0.1) 18.6 (± 0.4) 18.7 (± 0.2) 20.1 (± 0.3) 25.3 (± 1.3) 27.3 (± 0.2)
Loss/solubilization after HWPa
 Cellulose (%) 25.3 (± 1.5) 23.9 (± 5.3) 23.1 (± 1.0) 30.6 (± 0.8)
 Hemicelluloses (%) 13.4 (± 3.2) 19.6 (± 4.2) 50.5 (± 3.4) 45.7 (± 1.1)
 Klason lignin (%) 8.7 (± 1.0) 11.8 (± 1.1) 3 (± 0.4) 6.6 (± 0.7)
  1. Results are expressed as means of three repetitions with standard deviation in brackets
  2. a Loss/solubilization after HWP (%) = 100 × [1 − MY × (Yf/Yi)], where MY is the mass yield of the HWP, Yf is the final content and Yi is the initial content (raw CWR)