Fig. 2
From: CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars
Minimal medium development for T. aurantiacus for performing RNA-Seq under fed-batch conditions. A, B Fungal growth characteristics in McClendon’s salts supplemented with soy meal peptone (SMP) or ammonium sulfate (AmSO4). Enzyme production in the new growth medium (25 mM AmSO4 and 25 mM sodium citrate) was assessed through fed-batch feeding of d-xylose, cellobiose and l-arabinose and tested with C SDS-PAGE (20 μl filtered supernatant broth loaded per lane), D Bradford Protein Assay and E DNS-xylanase assay (error bars represent standard deviations, n = 3)