Rational engineering of Saccharomyces cerevisiae towards improved tolerance to multiple inhibitors in lignocellulose fermentations

Table 2 Performance of the best transformant per gene combination after 1st stage of strain development

Gene combination	Strain	Resistance phenotype	EtOH yield % increment	Growth (OD₆₀₀) % increment
ARI + ADH6	AA6	Furans + weak acids	15.80	19.50
ARI1 + FDH1	AF10	Furans + weak acids	− 5.55	7.92
ARI1 + ICT1	AI1	Furans + weak acids + organic solvents	1.25	− 12.00
PAD1 + ADH6	PA7	Phenolics + furans + weak acids	− 1.97	3.40
PAD1 + FDH1	PF5	Phenolics + weak acids	− 15.40	31.80
PAD1 + ICT1	PI3	Phenolics + weak acids + organic solvents	− 3.58	20.90
TAL1 + ADH6	TA6	Furans + weak acids	3.60	18.60
TAL1 + FDH1	TF2	Weak acids	16.90	6.20
TAL1 + ICT1	TI10	Weak acids + organic solvents	− 8.34	− 22.30

ISSN: 2731-3654