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Table 3 Fermentation kinetic parameters of recombinant S. cerevisiae strains and control strains

From: Rational engineering of Saccharomyces cerevisiae towards improved tolerance to multiple inhibitors in lignocellulose fermentations

Strain

Glucose cons. %

Xylose cons. %

Ethanol

Metabolic yield %

Media pH

µMax (h−1)

  

g L−1

Y P/S g g−1

g L−1 h−1

   

YPDX t=24 h

       

 CelluXTM1

100

100

17.0 ± 0.13

0.424 ± 0.00

0.707 ± 0.01

82.8

5

0.572

 TFA7

100

100

17.1 ± 0.13

0.428 ± 0.00

0.713 ± 0.01

83.5

5

0.557

 AP1

100

45.7

12.2 ± 0.09

0.305 ± 0.00

0.508 ± 0.00

82.1

5

0.513

 TP1

100

100

17.3 ± 0.08

0.432 ± 0.00

0.720 ± 0.00

84.4

5

0.545

YPX t=24 h

       

 CelluXTM1

–

100

8.18 ± 0.05

0.396 ± 0.02

0.341 ± 0.00

77.7

5

0.412

 TFA7

–

90.1

7.52 ± 0.25

0.364 ± 0.01

0.313 ± 0.01

78.3

5

0.364

 AP1

–

42.3

3.81 ± 0.02

0.184 ± 0.00

0.159 ± 0.00

73.7

5

0.442

 TP1

–

88.5

7.47 ± 0.35

0.362 ± 0.02

0.311 ± 0.01

78.9

5

0.372

40% v/v SSL t=72 h

       

 CelluXTM1

100

5.8

11.6 ± 0.93

0.228 ± 0.00

0.161 ± 0.01

–

5

–

 TFA7

100

3.0

11.7 ± 0.71

0.225 ± 0.01

0.162 ± 0.01

–

5

–

 AP1

100

0

11.8 ± 1.06

0.239 ± 0.00

0.160 ± 0.02

–

5

–

 TP1

100

1.28

12.2 ± 1.17

0.255 ± 0.03

0.169 ± 0.02

–

5

–

80% v/v SSL t=241 h

       

 CelluXTM1

15.1

1.74

0.51 ± 0.03

n.d.a

–

–

4.5

–

 TFA7

15.8

1.88

0.66 ± 0.03

n.d.a

–

–

4.5

–

 AP1

10.6

0.76

n.d.a

–

–

–

4.5

–

 TP1

16.3

1.55

0.48 ± 0.05

n.d.a

–

–

4.5

–

  1. aNot detected/determined
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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