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Table 5 Final plasmids and yeast strains used in study

From: Rational engineering of Saccharomyces cerevisiae towards improved tolerance to multiple inhibitors in lignocellulose fermentations

Plasmids/strain Relevant genotype References
Plasmids
 pBKD1 bla δ-site PGK1P-PGK1T kanMX δ-site [52]
 pBKD-AA bla δ-site PGK1P-ARI1-PGK1T kanMX ENO1P-ADH6-ENO1T δ-site This work
 pBHD1 bla δ-site PGK1P-PGK1T hphNT δ-site [53]
 pBHD-TF bla δ-site PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T δ-site This work
 pBZD1 bla δ-site PGK1P-PGK1T ShBle δ-site [52]
 pBZD-PI bla δ-site PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T δ-site This work
S. cerevisiae strains
S. cerevisiae CelluXTM1 BUD5::ADH1P-XKS1 -CYC1T TAL1::PGK1P-TAL1-CYC1T TKLI::TDH3P-TKL1-CYC1T RPE1::TDH3P-RPE1-CYC1T RKI1::TDH3P-RKI1-CYC1T HO::PsXYLZ-HYGRO BUD5::CpXI-BLAST GRE3/∆GRE3 [19]
S. cerevisiae AA6 CelluXTM1 FPS1/∆FPS1 PGK1P-ARI1-PGK1T kanMX ENO1P-ADH6-ENO1T This work
S. cerevisiae TF2 CelluXTM1 FPS1/∆FPS1 PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T This work
S. cerevisiae PI3 CelluXTM1 FPS1/∆FPS1 PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T This work
S. cerevisiae TFA7 CelluXTM1 FPS1/∆FPS1 PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T PGK1P-ARI1-PGK1T kanMX ENO1P-ADH6-ENO1T This work
S. cerevisiae TP1 TFA7 PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T This work
S. cerevisiae AP1 AA6 PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T This work