Table 5 Final plasmids and yeast strains used in study
Plasmids/strain | Relevant genotype | References | ||
|---|---|---|---|---|
Plasmids | ||||
pBKD1 | bla δ-site PGK1P-PGK1T kanMX δ-site | [52] | ||
pBKD-AA | bla δ-site PGK1P-ARI1-PGK1T kanMX ENO1P-ADH6-ENO1T δ-site | This work | ||
pBHD1 | bla δ-site PGK1P-PGK1T hphNT δ-site | [53] | ||
pBHD-TF | bla δ-site PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T δ-site | This work | ||
pBZD1 | bla δ-site PGK1P-PGK1T ShBle δ-site | [52] | ||
pBZD-PI | bla δ-site PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T δ-site | This work | ||
S. cerevisiae strains | ||||
S. cerevisiae CelluXTM1 | BUD5::ADH1P-XKS1 -CYC1T TAL1::PGK1P-TAL1-CYC1T TKLI::TDH3P-TKL1-CYC1T RPE1::TDH3P-RPE1-CYC1T RKI1::TDH3P-RKI1-CYC1T HO::PsXYLZ-HYGRO BUD5::CpXI-BLAST GRE3/∆GRE3 | [19] | ||
S. cerevisiae AA6 | CelluXTM1 FPS1/∆FPS1 PGK1P-ARI1-PGK1T kanMX ENO1P-ADH6-ENO1T | This work | ||
S. cerevisiae TF2 | CelluXTM1 FPS1/∆FPS1 PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T | This work | ||
S. cerevisiae PI3 | CelluXTM1 FPS1/∆FPS1 PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T | This work | ||
S. cerevisiae TFA7 | CelluXTM1 FPS1/∆FPS1 PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T PGK1P-ARI1-PGK1T kanMX ENO1P-ADH6-ENO1T | This work | ||
S. cerevisiae TP1 | TFA7 PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T | This work | ||
S. cerevisiae AP1 | AA6 PGK1P-TAL1-PGK1T hphNT ENO1P-FDH1-ENO1T PGK1P-PAD1-PGK1T ShBle ENO1P-ICT1-ENO1T | This work | ||