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Table 2 Hydrolysis yields and glucose isomerization to fructose for different pretreated biomass samples

From: Conversion of organosolv pretreated hardwood biomass into 5-hydroxymethylfurfural (HMF) by combining enzymatic hydrolysis and isomerization with homogeneous catalysis

Biomass No

Glucose (g/L)

Cellulose to glucose conversion (%)

Glucose to fructose conversion (%)

g fructose/g cellulose

g fructose/100 g pretreated biomass

ACΟ/H2O

 1

59.91

(0.49)

77.98

(0.64)

78.80

(0.14)

0.61

(0.10)

38.00

(1.66)

 2

87.78

(2.22)

92.79

(2.34)

79.87

(0.13)

0.74

(0.10)

56.79

(1.34)

 3

68.17

(2.15)

82.71

(2.61)

76.34

(0.16)

0.63

(0.16

42.16

(2.36)

 4

80.16

(0.22)

81.44

(0.22)

77.23

(0.15)

0.63

(0.13)

50.15

(1.60)

 5

71.97

(0.99)

69.99

(0.97)

75.00

(0.17)

0.51

(0.13)

42.38

(1.54)

 6

71.58

(0.22)

67.34

(0.21)

73.68

(0.18)

0.50

(0.12)

42.72

(1.42)

 7

78.06

(0.99)

76.83

(0.98)

78.58

(0.14)

0.60

(0.10)

49.68

(1.23)

 8

42.78

(0.37)

56.25

(0.48)

68.12

(0.21)

0.38

(0.12)

23.61

(1.88)

EtOH/H2O

 

33.73

(0.00)

50.39

(0.00)

71.53

(0.00)

0.36

(0.00)

19.55

(0.00)

 9

55.38

(0.25)

79.06

(0.35)

74.38

(0.17)

0.59

(0.14)

33.37

(2.45)

 10

80.26

(1.18)

89.11

(1.31)

76.30

(0.16)

0.68

(0.13)

49.61

(1.78)

 11

48.37

(1.63)

59.02

(1.32)

72.37

(0.68)

0.43

(0.01)

28.36

(1.84)

 12

84.59

(1.06)

88.88

(1.11)

75.94

(0.17)

0.64

(0.16)

49.36

(2.03)

 13

82.23

(2.65)

80.67

(2.15)

73.13

(0.44)

0.59

(0.02)

48.70

(2.41)

 14

86.08

(0.56)

92.21

(0.45)

79.05

(0.09)

0.73

(0.00)

55.15

(0.55)

THF/H2O

 15

79.51

(0.12)

93.36

(0.14)

80.11

(0.13)

0.75

(0.13)

51.60

(1.83)

 16

78.70

(1.81)

80.57

(1.85)

78.19

(0.14)

0.63

(0.10)

49.85

(1.27)

 17

57.60

(0.32)

71.49

(0.40)

74.14

(0.17)

0.53

(0.12)

34.59

(1.84)

 18

83.27

(2.58)

79.10

(2.45)

79.12

(0.14)

0.63

(0.09)

53.37

(1.02)

 19

78.98

(0.84)

75.09

(0.68)

70.80

(0.23)

0.53

(0.01)

45.28

(0.74)

 20

79.47

(1.54)

84.31

(1.24)

78.97

(0.26)

0.67

(0.01)

50.81

(1.51)

 Untreated

12.86

(0.19)

25.97

(0.15)

70.89

(0.31)

0.18

(0.00)

7.38

(0.35)

 Avicel®

76.05

(0.26)

64.51

(0.21)

80.40

(0.11)

0.50

(0.08)

48.21

(0.81)

  1. Numbers in parenthesis represent the standard error values
  2. All experiments were run in duplicates and numbers represent the mean values