Table 1 Plasmids and strains used in the study
| Name | Genotype | Sources |
|---|---|---|
| Plasmids | ||
| pAM2991 | NSI targeting vector Ptrc; Specr | Addgene #40248 |
| pJW11 | Specr; NSI targeting; Ptrc::Kivd, adhA | This study |
| pJW12 | Specr; NSI targeting; Ptrc::Kivd, adhA, PLlacO1:alsS, ilvC, ilvD | This study |
| Relevant genotypes | ||
|---|---|---|
| Strains | ||
| S. elongatus | Wide type | ATCC #33912 |
| JW10 | pAM2991 integrated at NSI in S. elongatus genome | This study |
| JW11 | pJW12 integrated at NSI in S. elongatus genome | This study |