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Table 1 Plasmids and strains used in the study

From: Establishment of a resource recycling strategy by optimizing isobutanol production in engineered cyanobacteria using high salinity stress

Name

Genotype

Sources

Plasmids

 pAM2991

NSI targeting vector Ptrc; Specr

Addgene #40248

 pJW11

Specr; NSI targeting; Ptrc::Kivd, adhA

This study

 pJW12

Specr; NSI targeting; Ptrc::Kivd, adhA, PLlacO1:alsS, ilvC, ilvD

This study

 

Relevant genotypes

 

Strains

 S. elongatus

Wide type

ATCC #33912

 JW10

pAM2991 integrated at NSI in S. elongatus genome

This study

 JW11

pJW12 integrated at NSI in S. elongatus genome

This study