Fig. 9

Highly conserved area containing π–π interactions in GH53 galactanases. A Superposition of the conserved aromatic residues localized in the 6th β/α-loop. The residues from IaGal are labelled and shown in green, BlGal is shown in magenta and all the fungal galactanases are shown in cyan. Unique for BlGal and IaGal is the extension of the aromatic cluster with an additional histidine residue. B An overview of the π–π cluster in IaGal. The residues located in the 6th β/α-loop are shown as greens sticks in the same orientation as in A. Adjacent aromatic residues which interact with these conserved residues are shown as orange sticks (1: Phe247, 2: Tyr269, 3: Phe283, 4: Trp346)