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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield

Fig. 1

Illustration of the strategy for reducing lignin content by targeting the AtCCR1 locus using a sgRNA/Cas9 design while restoring CCR1 function to vascular tissues. a Diagram of CRISPR/Cas9 system with a sgRNA that targets Cas9 to the native CCR1 gene. b Construct for vessel-specific expression of codon-modified CCR1 that encodes the same protein as native CCR1, driven by the xylem vessel-specific promoter SNBE. c Final construct for inserting the ProSNBE: artificial CCR1 into the pCambia 1300-CAS9 vector with the sgRNA designed for targeting the native CCR1

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