Fig. 2

Genotyping of T1 transgenic plants and the distribution of zygosity. a Zygosity of T1‐edited plants. Following isolation of leaf DNA, genomic sequences spanning the sgRNA target site were PCR amplified and analyzed by Sanger sequencing. Transgenic plants of each line were classified based on mutation rate at the projected target site. b Target sequences of wild-type Col-0 and representative homozygous ccr1 mutants in Cas9 transgenic lines. Their detection frequencies and the indel patterns (red color) are shown, and the sgRNA (underlined) and protospacer adjacent motif (PAM; bold text) sequences are highlighted. c Analysis scheme for gene-edited lines. For 12-week-old T1 homozygous and bi-allele ccr1 mutants, seeds were harvested from individual seedlings, the bottom 5 cm of inflorescence stems were taken for imaging of cross-sections for lignin deposition, and the main regions of inflorescence stems were harvested for lignin measurement and sugar release assay