Table 1 Comparison of studies on [FeFe] hydrogenase genes expression in various conditions available in literature and the present study
Organism(s) | Methodological approach | Growth conditions | Focused metabolism studied | [FeFe] hydrogenase genes expression monitored | Refs. |
|---|---|---|---|---|---|
C. beijerinckii SM10 | Semi-quantitative RT-PCR | Clostridial medium, pure culture | Hydrogen production | Cbei_1773 Cbei_0327 Cbei_1901 Cbei_4110 Cbei_3796 Cbei_4000 | [35] |
C. butyricum SM32 | Semi-quantitative RT-PCR | Clostridial medium, pure culture | Hydrogen production | CBY_3049 CBY_2300 CBY_2047 CBY_2676 | [35] |
C. perfringens SM09 | Semi-quantitative RT-PCR | Clostridial medium, pure cultures | Hydrogen production | CPF_2655 CPF_1076 CPF_0270 CPF_2900 | [35] |
C. tyrobutyricum KCTC 5387 (ATCC 25,755) | Shotgun proteomics | 2xYTG medium Pure culture | General metabolism | CTK_C05160 CTK_C26290 CTK_C29580 | [43] |
Proteobacteria, Firmicutes, Bacteroidetes, and Chloroflexi | DGGE analysis of hydA in time | Paddy Field Soil during rice straw decomposition with autochthonous microbiota | Hydrogen production | hydA sequences of uncultured strains deposited to the DDBJ database under accession numbers LC041370-LC041941 | [45] |
Clostridium beijerinckii NRRL B-598 | RNA-Seq | Batch fermentation, pure culture in culture broths with a glucose concentration of 50Â g/L at pH 6.3 | Acidogenesis, solventogenesis, metabolic stress response and life cycle changes | X276_17350, X276_06930 X276_05300 (sharing high similarity with Cbei_1901, Cbei_3796 and Cbei_4110, respectively) | [46] |
Clostridium butyricum CWBI 1009 | Transcriptomic (RNA-seq and RT-qPCR) and proteomic analyses | Glucose fermentation, unregulated pH with Argon atmosphere pure culture | Hydrogen production | hydA2, hydA8 hydB2 hydB3 (changes only observed in Argon atmosphere, not in N2) | [49] |
Clostridium sp. strain H2 | RT-qPCR | Under fermentation conditions, Widdel’s freshwater medium pure cultures | Hydrogen production | H2hydA1 H2hydA2 H2hydA3 H2hydA4 H2hydA5 corresponding to hydA genes of C. bifermentans ATCC 638 (AVNC00000000) and ATCC 19,299 (AVNB00000000) | [50] |
Desulfovibrio sp. strain A1 and AH1 | RT-qPCR | Under sulphate-reducing conditions modified Widdel’s freshwater medium pure cultures | Hydrogen production | A1hydA1 A1hydA2 corresponding to hydA genes of D. vulgaris Hildenborough (AE017285) | [50] |
Clostridium butyricum CGS5 | RT-PCR and qPCR | PM medium Pure culture | Hydrogen production | hydA | [51] |
Thermotoga neapolitana and Caldicellulosiruptor saccharolyticus | Quantitative Real-time PCR | Synthetic co-culture | Hydrogen production | hydA from Thermotoga neapolitana | [52] |
Clostridium thermocellum ATCC 27,405 | Real-time quantitative PCR | Continuous pure culture on Modified Dehority medium containing (per litre) 3.0Â g of cellobiose or 2.7 to 3.1Â g of Sigmacell 20 microcrystalline cellulose | Fermentation of Cellulose or Cellobiose | hydA | [53] |
Clostridium beijerinckii AM2 and Clostridium tyrobutyricum AM6 | RT-qPCR | Green waste with autochthonous microbiota and co-addition of both strains | Hydrogen production on green waste | Cbei_1773 Cbei_0327 Cbei_1901 Cbei_4110 Cbei_3796 Cbei_4000 Ctyr_hydA (FJ226584) | This study |