Table 2 List of primers, amplicon fragment size and features
Gene description | Forward (5′-3′) | Reverse (5′-3′) | Size (bp) | E% | R2 | Tm (℃) |
|---|---|---|---|---|---|---|
Ctyr_recA | GTGGAAGCATTGGTTAGATC | GTCCTACATGAGAATCTCCCA | 111 | 101 | 0.98 | 75.1 |
Cbei_recA | GTCACAGGCGTTAAGAAAGCT | TGCTCTTCCTCCAGTTGTTG | 129 | 87.5 | 0.99 | 74.5 |
Cbei_1773 | GAGGTTGGGATGGATTCAGA | CCACCTACACACGCCATTA | 161 | 89.3 | 0.99 | 75.6 |
Cbei_0327 | AGCCTTATTCGATGCGTTTG | TCAATCCACCACCTACAGCA | 104 | 95.9 | 0.99 | 74.8 |
Cbei_1901 | TTGTGGTGTATGCGTGGATT | AGTACCAAATTGCCCGCTA | 139 | 88.0 | 0.99 | 74.4 |
Cbei_4000 | AGGGTGGCATAAATGGAGGTG | CCTCTGCCCTTCCTCTCTAACA | 140 | 97.1 | 0.99 | 75.3 |
Cbei_4110 | TGGTGATTGATGGCAATAGG | TCATCCTCGACCACACACAT | 148 | 95.2 | 0.99 | 73.3 |
Cbei_3796 | TGTTTGCGTTTCTTGTGGAC | TGGCTCCTTCACACTCTCAA | 127 | 103 | 0.98 | 72.7 |
Ctyr_hydA′ | CCATGCCCAAGAAGAGAAAA | GCATTTGGTTCTGTCAAGCA | 144 | 88.0 | 0.99 | 75.0 |
Ctyr_hydA″ | GAGGCAAATGGCAGAACAAT | TTTTCTCTTCTTGGGCATGG | 161 | 95.2 | 0.99 | 74.7 |