Fig. 5

GH activities from P. sumatraense AQ67100 cultures upon growth in different cell wall polysaccharide-supplemented media. a Analysis of GH activities in the filtrates from different cell wall polysaccharide-supplemented cultures upon 10 days of growth. B-medium was supplemented with 0.5% (w/v) arabinoxylan (AX), 0.5% (w/v) xyloglucan (XG), 0.2% (w/v) heat-treated C. vulgaris biomass (C.v.) and 0.2% (w/v) C. vulgaris AIS (C.v. AIS). B-medium without any carbon source (NS) was used as negative control. The corresponding cultures are shown below the graph. Units are expressed as µmol reducing ends (for endo-β-1,4-glucanase, xyloglucanase, arabinoxylanase and endo-β-1,3-glucanase activities) and µmol pnitrophenol (for β-glucosidase, β-galactosidase and exo-β-1,3-glucanase activities) released per minute. Experiments were repeated in triplicate with consistent results. B SDS-PAGE analysis carried out on the same filtrates assayed in a. AX and NS filtrates were analysed as positive and negative control of CWDE-production, respectively. The molecular weight marker (MM) is also reported