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Table 4 Proteins identified in AX-, C.v.- and C.v. AIS-filtrates by LC–MS/MS analysis

From: A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes

Seq. cov. [%] Unique peptides MW [kDa] Protein ID Predicted protein function
59 13 47.023 g9356.t1 Chitinase
58.9 12 40.258 g10009.t1 Tri m 2 allergen
50.5 20 78.135 g4340.t1 β-glucosidase L
47.4 17 56.436 g1651.t1 α-1,2-mannosidase
43.9 22 71.912 g4150.t1 β-glucuronidase
43.6 24 80.724 g184.t1 Catalase
43.1 11 50.366 g5037.t1 Phosphoesterase
42.1 24 80.26 g4775.t1 Secreted dipeptidyl peptidase
41.4 14 52.692 g1317.t1 Aminopeptidase
39.7 10 36.195 g1453.t1 Endo-arabinase
38.4 15 70.144 g10362.t1 β-Glucosidase C
37.3 16 59.742 g10336.t1 α-Galactosidase A
36.9 15 69.492 g1388.t1 α-l-Arabinofuranosidase
35.4 6 32.237 g6686.t1 Necrosis inducing
35.1 14 54.255 g6152.t1 Glycoside hydrolase family 43
34.5 11 43.973 g11244.t1 Chitinase
34.5 11 46.298 g11932.t1 Phosphoesterase
31.8 11 42.947 g11298.t1 Pectinesterase
30.1 11 61.951 g9290.t1 Carboxylic ester hydrolase
29.4 8 52.517 g11560.t1 1,4-α-Glucosidase
29.1 22 93.387 g484.t1 Scopolin β-glucosidase
28.9 12 68.34 g1853.t1 β-galactosidase
28.7 10 58.956/58.664 g8894.t1/t2 Carboxylic ester hydrolase
28.4 20 106.2 g10014.t1 α-glucosidase B
28.3 15 93.568 g9771.t1 α-1,2-mannosidase
28.3 14 85.189 g9376.t1 Exo-β-1,3-glucanase
28.2 24 105.2 g7401.t1 Aminopeptidase
28.2 11 65.349 g12386.t1 Tripeptidyl-peptidase
27.7 12 75.751 g5343.t1 Glutaminase GtaA
27.4 8 51.894 g3293.t1 α-l-Rhamnopyranohydrolase
24.6 15 103.1 g5736.t1 Exo-β-1,3-glucanase Exg0
24.6 7 34.711 g11633.t1 Endo-1,4-β-xylanase C
24.3 8 52.419 g411.t1 α-l-Arabinofuranosidase
24.1 16 109.98 g6118.t1 β-galactosidase A
23.9 11 70.86 g8605.t1 Six-hairpin glycosidase
23.9 6 49.312 g7048.t1 Glucan endo-1,3-β-glucosidase eglC
23.8 12 66.558 g3213.t1 β-N-Acetylhexosaminidase
23.3 8 49.119 g7605.t1 1,3-β-Glucanosyltransferase
23 10 70.285 g6452.t1 Phosphoesterase-domain-containing protein
22.9 9 61.407 g4908.t1 Carboxylic ester hydrolase
22.8 9 59.375 g10235.t1 Meiotically up-regulated gene 157 protein
22.2 10 64.929 g330.t1 Serine-type carboxypeptidase
21.9 7 47.965 g10066.t1 Exo-polygalacturonase B
21.8 14 96.628 g4584.t1 α-l-Rhamnosidase
21.5 10 72.621 g1190.t1 O-GlcNAcase NagJ
21.4 6 67.869/68.096 g10052.t1/t2 Glucoamylase GLAA
21.2 14 85.962 g3950.t1 Exo-1,4-β-xylosidase xlnD
20.4 9 62.198 g8900.t1 Carboxypeptidase
19.1 6 42.831 g9323.t1 Endo-1,4-β-xylanase
18.2 10 87.891 g9296.t1 Glycosyl hydrolase family 92
  1. The sequence coverage (percentage of the protein sequence covered by identified peptides), unique peptides (number of peptide sequences that are unique to a protein), the protein ID, the expected molecular weight and the predicted protein function are reported for each identified protein. Proteins are listed in decreasing order of sequence coverage. Only proteins identified with the highest score (323.31) are listed. Identification was performed by using the annotated genome of P. sumatraense AQ67100 as reference database. Proteins specifically identified in AX-filtrate are highlighted in bold. All the remaining proteins were identified in AX- and at least in 1 out of 2 filtrates from algal-supplemented cultures. See Fig. 6 for further details on identified proteins