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Table 4 Proteins identified in AX-, C.v.- and C.v. AIS-filtrates by LC–MS/MS analysis

From: A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes

Seq. cov. [%]

Unique peptides

MW [kDa]

Protein ID

Predicted protein function

59

13

47.023

g9356.t1

Chitinase

58.9

12

40.258

g10009.t1

Tri m 2 allergen

50.5

20

78.135

g4340.t1

β-glucosidase L

47.4

17

56.436

g1651.t1

α-1,2-mannosidase

43.9

22

71.912

g4150.t1

β-glucuronidase

43.6

24

80.724

g184.t1

Catalase

43.1

11

50.366

g5037.t1

Phosphoesterase

42.1

24

80.26

g4775.t1

Secreted dipeptidyl peptidase

41.4

14

52.692

g1317.t1

Aminopeptidase

39.7

10

36.195

g1453.t1

Endo-arabinase

38.4

15

70.144

g10362.t1

β-Glucosidase C

37.3

16

59.742

g10336.t1

α-Galactosidase A

36.9

15

69.492

g1388.t1

α-l-Arabinofuranosidase

35.4

6

32.237

g6686.t1

Necrosis inducing

35.1

14

54.255

g6152.t1

Glycoside hydrolase family 43

34.5

11

43.973

g11244.t1

Chitinase

34.5

11

46.298

g11932.t1

Phosphoesterase

31.8

11

42.947

g11298.t1

Pectinesterase

30.1

11

61.951

g9290.t1

Carboxylic ester hydrolase

29.4

8

52.517

g11560.t1

1,4-α-Glucosidase

29.1

22

93.387

g484.t1

Scopolin β-glucosidase

28.9

12

68.34

g1853.t1

β-galactosidase

28.7

10

58.956/58.664

g8894.t1/t2

Carboxylic ester hydrolase

28.4

20

106.2

g10014.t1

α-glucosidase B

28.3

15

93.568

g9771.t1

α-1,2-mannosidase

28.3

14

85.189

g9376.t1

Exo-β-1,3-glucanase

28.2

24

105.2

g7401.t1

Aminopeptidase

28.2

11

65.349

g12386.t1

Tripeptidyl-peptidase

27.7

12

75.751

g5343.t1

Glutaminase GtaA

27.4

8

51.894

g3293.t1

α-l-Rhamnopyranohydrolase

24.6

15

103.1

g5736.t1

Exo-β-1,3-glucanase Exg0

24.6

7

34.711

g11633.t1

Endo-1,4-β-xylanase C

24.3

8

52.419

g411.t1

α-l-Arabinofuranosidase

24.1

16

109.98

g6118.t1

β-galactosidase A

23.9

11

70.86

g8605.t1

Six-hairpin glycosidase

23.9

6

49.312

g7048.t1

Glucan endo-1,3-β-glucosidase eglC

23.8

12

66.558

g3213.t1

β-N-Acetylhexosaminidase

23.3

8

49.119

g7605.t1

1,3-β-Glucanosyltransferase

23

10

70.285

g6452.t1

Phosphoesterase-domain-containing protein

22.9

9

61.407

g4908.t1

Carboxylic ester hydrolase

22.8

9

59.375

g10235.t1

Meiotically up-regulated gene 157 protein

22.2

10

64.929

g330.t1

Serine-type carboxypeptidase

21.9

7

47.965

g10066.t1

Exo-polygalacturonase B

21.8

14

96.628

g4584.t1

α-l-Rhamnosidase

21.5

10

72.621

g1190.t1

O-GlcNAcase NagJ

21.4

6

67.869/68.096

g10052.t1/t2

Glucoamylase GLAA

21.2

14

85.962

g3950.t1

Exo-1,4-β-xylosidase xlnD

20.4

9

62.198

g8900.t1

Carboxypeptidase

19.1

6

42.831

g9323.t1

Endo-1,4-β-xylanase

18.2

10

87.891

g9296.t1

Glycosyl hydrolase family 92

  1. The sequence coverage (percentage of the protein sequence covered by identified peptides), unique peptides (number of peptide sequences that are unique to a protein), the protein ID, the expected molecular weight and the predicted protein function are reported for each identified protein. Proteins are listed in decreasing order of sequence coverage. Only proteins identified with the highest score (323.31) are listed. Identification was performed by using the annotated genome of P. sumatraense AQ67100 as reference database. Proteins specifically identified in AX-filtrate are highlighted in bold. All the remaining proteins were identified in AX- and at least in 1 out of 2 filtrates from algal-supplemented cultures. See Fig. 6 for further details on identified proteins