Fig. 1
From: Co-production of hydrogen and ethyl acetate in Escherichia coli
Schematic representation of anaerobic ethyl acetate production from glucose in E. coli via the Embden–Meyerhof–Parnas (EMP) pathway with hydrogen co-production. Lactate and acetate formation is limited by ack and ldh inactivation. Heterologous alcohol acetyltransferase Eat1 generates ethyl acetate from ethanol and acetyl-CoA. Hydrogen co-production is achieved via formate hydrogen lyase (Fhl). Ack acetate kinase, DHAP dihydroxyacetone phosphate, eat1 ethanol acetyltransferase, FBR fructose 1,6-bisphosphate, F6P fructose 6-phosphate, G6P glucose 6-phosphate, PEP phosphoenolpyruvate, GAP glyceraldehyde 3-phosphate, pta phosphate acetyltransferase