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Table 1 Strains used in this study

From: Co-production of hydrogen and ethyl acetate in Escherichia coli

Strain

Characteristics

Source

Escherichia coli BW25113 (DE3)

Wild type with integrated DE3 lysogen

[32]

Escherichia coli BW25113 ΔackAΔldhA

Disruption of lactate and acetate production (via ackA)

[9]

Escherichia coli BW25113 ΔackAΔldhA p3-fhlA

Disruption of lactate and acetate production (via ackA) and overexpression of formate hydrogen lyase transcriptional activator (fhlA)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhycA

Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhyaAB

Disruption of lactate and acetate production (via ackA) and inactivation of uptake hydrogenase (hyaAB)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhybBC

Disruption of lactate and acetate production (via ackA) and inactivation of uptake hydrogenase (hybBC)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhyaAB

Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenase (hyaAB)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhybBC

Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenase (hybBC)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhyaABΔhybBC

Disruption of lactate and acetate production (via ackA) and inactivation of uptake hydrogenases (hyaAB, hybBC)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhyaABΔhybBC

Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenases (hyaAB and hybBC)

This study

Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhyaABΔhybBC p3-fhlA

Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenases (hyaAB and hybBC) with overexpression of Fhl activator (fhlA)

This study

Escherichia coli T7 Express

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS

λ DE3 = λ sBamHIo ∆EcoRI-B int::(LacI::PlacUV5::T7 gene1) i21 ∆nin5

NEB

Escherichia coli NEB® 5-alpha

fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

NEB