From: Co-production of hydrogen and ethyl acetate in Escherichia coli
Strain | Characteristics | Source |
---|---|---|
Escherichia coli BW25113 (DE3) | Wild type with integrated DE3 lysogen | [32] |
Escherichia coli BW25113 ΔackAΔldhA | Disruption of lactate and acetate production (via ackA) | [9] |
Escherichia coli BW25113 ΔackAΔldhA p3-fhlA | Disruption of lactate and acetate production (via ackA) and overexpression of formate hydrogen lyase transcriptional activator (fhlA) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhycA | Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhyaAB | Disruption of lactate and acetate production (via ackA) and inactivation of uptake hydrogenase (hyaAB) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhybBC | Disruption of lactate and acetate production (via ackA) and inactivation of uptake hydrogenase (hybBC) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhyaAB | Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenase (hyaAB) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhybBC | Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenase (hybBC) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhyaABΔhybBC | Disruption of lactate and acetate production (via ackA) and inactivation of uptake hydrogenases (hyaAB, hybBC) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhyaABΔhybBC | Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenases (hyaAB and hybBC) | This study |
Escherichia coli BW25113 ΔackAΔldhAΔhycAΔhyaABΔhybBC p3-fhlA | Disruption of lactate and acetate production (via ackA) and inactivation of Fhl repressor (hycA) and uptake hydrogenases (hyaAB and hybBC) with overexpression of Fhl activator (fhlA) | This study |
Escherichia coli T7 Express | fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(LacI::PlacUV5::T7 gene1) i21 ∆nin5 | NEB |
Escherichia coli NEB® 5-alpha | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NEB |