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Table 2 Plasmids used in this study

From: Co-production of hydrogen and ethyl acetate in Escherichia coli

Plasmid

Promoter

Gene/protein

Source

pET26b

LacI/T7

–

This study

pET26b:hWan trEat1 N-13

LacI/T7

Codon-harmonized eat1 from Wickerhamomyces anomalus DSM 6766

[9]

pCas9

–

 

[5]

pTarget

–

 

[5]

pTarget-hycA

–

 

This study

pTarget-hyaAB

–

 

This study

pTarget-hybBC

–

 

This study

pTarget-p3

–

 

This study

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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