Table 2 Fatty acid composition in S. elongatus expressing the empty vector pSyn6, CzFAD2, CzFAD6A or CzFAD6B without or with FFA feeding
Fatty acids (%) | Without FFA feeding | With C16:1Δ9 feeding | With C18:1Δ9 feeding | With C18:1Δ11 feeding | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
pSyn6 | CzFAD2 | CzFAD6A | CzFAD6B | pSyn6 | CzFAD2 | CzFAD6A | CzFAD6B | pSyn6 | CzFAD2 | CzFAD6A | CzFAD6B | pSyn6 | CzFAD2 | CzFAD6A | CzFAD6B | |
C16:0 | 44.5 ± 3.4 | 42.7 ± 2.9 | 40.7 ± 2.1 | 44.9 ± 1.1 | 35.3 ± 1.1 | 33.5 ± 2.2 | 30.4 ± 2.5 | 38.1 ± 2.6 | 35.2 ± 2.0 | 35.0 ± 1.7 | 36.5 ± 1.0 | 36.2 ± 3.0 | 35.9 ± 3.0 | 33.4 ± 2.0 | 30.7 ± 2.0 | 36.5 ± 1.9 |
C16:1Δ9 | 41.6 ± 2.3 | 34.6 ± 1.9 | 32.7 ± 1.4 | 40.3 ± 1.0 | 46.8 ± 3.2 | 39.7 ± 2.0 | 35.3 ± 1.8 | 44.9 ± 1.8 | 32.8 ± 1.3 | 29.8 ± 1.1 | 28.6 ± 2.9 | 33.2 ± 1.7 | 33.6 ± 3.0 | 27.2 ± 2.2 | 27.6 ± 2.3 | 30.9 ± 3.0 |
C16:2Δ9,12 | – | 3.4 ± 0.4 | 6.4 ± 1.0 | – | – | 8.1 ± 0.9 | 10.5 ± 0.8 | – | – | 4.0 ± 0.9 | 6.1 ± 0.3 | – | – | 3.3 ± 1.0 | 5.1 ± 1.0 | – |
C18:0 | 1.2 ± 0.0 | 1.3 ± 0.1 | 1.3 ± 0.3 | 1.7 ± 0.2 | 1.7 ± 0.3 | 1.2 ± 0.1 | 0.9 ± 0.0 | 1.4 ± 0.3 | 1.5 ± 0.0 | 1.5 ± 0.1 | 1.1 ± 0.1 | 1.3 ± 0.3 | 1.0 ± 0.3 | 1.1 ± 0.3 | 1.0 ± 0.0 | 1.4 ± 0.5 |
C18:1Δ9 | 4.4 ± 0.5 | 5.0 ± 1.0 | 4.8 ± 0.1 | 4.3 ± 1.0 | 4.0 ± 1.0 | 4.0 ± 0.8 | 4.1 ± 1.2 | 4.0 ± 0.8 | 23.7 ± 1.4 | 12.7 ± 2.6 | 10.3 ± 1.2 | 22.5 ± 2.7 | 3.5 ± 0.8 | 3.9 ± 0.1 | 3.8 ± 0.9 | 3.4 ± 1.0 |
C18:1Δ11 | 8.3 ± 1.0 | 8.3 ± 1.3 | 7.7 ± 0.3 | 8.7 ± 1.3 | 12.2 ± 1.0 | 9.6 ± 1.0 | 10.8 ± 1.6 | 11.6 ± 1.0 | 6.8 ± 0.7 | 7.5 ± 0.4 | 7.1 ± 1.1 | 6.8 ± 1.0 | 26.1 ± 2.3 | 27.5 ± 3.1 | 26.6 ± 1.1 | 27.9 ± 3.0 |
C18:2Δ9,12 | – | 2.9 ± 0.4 | 4.0 ± 0.9 | – | – | 2.5 ± 0.0 | 3.4 ± 0.4 | – | – | 5.9 ± 0.6 | 8.1 ± 1.3 | – | – | 2.3 ± 0.7 | 3.2 ± 0.2 | – |
C18:2Δ11,14 | – | 1.8 ± 0.1 | 2.4 ± 0.2 | – | – | 3.3 ± 0.1 | 4.6 ± 0.3 | – | – | 1.7 ± 0.1 | 2.2 ± 0.2 | – | – | 1.3 ± 0.0 | 1.9 ± 0.3 | – |