Table 3 Fatty acid composition in S. elongatus expressing the empty vector pSyn6, CzFAD7A or CzFAD7B without or with FFA feeding

C16:0

44.1 ± 1.6

48.5 ± 2.3

48.1 ± 3.0

40.9 ± 2.0

42.7 ± 1.0

44.8 ± 1.2

44.1 ± 2.1

41.3 ± 2.3

49.6 ± 3.1

41.5 ± 1.7

40.3 ± 2.2

47.9 ± 2.1

C16:1^Δ9

34.8 ± 2.4

41.8 ± 2.0

24.4 ± 1.2

39.0 ± 1.1

38.1 ± 2.2

18.7 ± 0.9

38.5 ± 2.2

27.0 ± 1.1

21.6 ± 1.6

44.1 ± 3.1

42.1 ± 3.7

20.3 ± 2.5

C16:2^Δ9,13

–

–

6.8 ± 1.0

–

–

4.4 ± 0.7

–

–

4.7 ± 0.6

–

–

4.6 ± 0.7

C18:0

2.1 ± 0.3

1.2 ± 0.2

4.9 ± 0.6

0.7 ± 0.0

0.6 ± 0.0

2.0 ± 0.1

1.9 ± 0.3

4.6 ± 0.9

5.8 ± 0.6

0.8 ± 0.0

0.9 ± 0.1

5.9 ± 1.1

C18:1^Δ9

7.5 ± 0.5

4.2 ± 0. 7

2.8 ± 0.3

13.5 ± 1.3

13.8 ± 1.2

7.3 ± 1.0

3.6 ± 0.2

5.2 ± 0.4

2.4 ± 0.4

2.9 ± 0.4

3.2 ± 1.0

2.3 ± 0.3

C18:1^Δ11

11.5 ± 1.0

4.2 ± 0.8

6.6 ± 1.0

5.9 ± 1.0

4.8 ± 0.7

2.1 ± 0.1

6.8 ± 0.9

15.7 ± 1.4

6.0 ± 0.8

4.6 ± 0.4

6.3 ± 1.2

6.9 ± 0.8

C18:2^Δ9,12

–

–

–

–

–

–

5.1 ± 0.7

6.2 ± 1.1

0.4 ± 0.0

–

–

–

C18:2^Δ9,15

–

–

2.8 ± 0.5

–

–

20.5 ± 2.5

–

–

1.5 ± 0.3

–

–

2.8 ± 0.3

C18:2^Δ11,15

–

–

3.6 ± 0.3

–

–

0.2 ± 0.0

–

–

2.9 ± 0.5

–

–

3.6 ± 1.1

C18:3^Δ6,9,12

–

–

–

–

–

–

–

–

–

6.1 ± 1.0

6.8 ± 1.2

2.9 ± 0.9

C18:3^Δ9,12,15

–

–

–

–

–

–

–

–

5.0 ± 0.5

–

–

–

C18:4^Δ6,9,12,15

–

–

–

–

–

–

–

–

–

–

–

2.8 ± 0.2