Fig. 4From: Alcohol dehydrogenases AdhE and AdhB with broad substrate ranges are important enzymes for organic acid reduction in Thermoanaerobacter sp. strain X514Cellular levels of ADHs in Thermoanaerobacter sp. strain X514 grown on glucose (three biological replicates, as indicated by left black bar), on glucose plus 25 mM isobutyrate (as indicated by middle black bar) or on glucose plus 50 mM isobutyrate (as indicated by right black bar). Cell-free extracts (1 μg) were separated via SDS-PAGE. The presence of AdhA, Adh0564, AdhE and AdhB, was determined immunologically with antibodies generated against the His-tagged, affinity-purified proteins. Left lanes, purified proteins as controlBack to article page