Metabolic engineering of the oleaginous yeast Yarrowia lipolytica PO1f for production of erythritol from glycerol

Table 1 The fermentation data for engineered strains versus wild type

Strain name	Erythritol (g/L)	Biomass (DCW g/L)	Time (h)	Qery (g/L h)	Yery (g/g glycerol)	R gly (g/L/h)	CP ery (g/g dcw h)
Yarrowia lipolytica O1f	10.70 ± 0.20	8.52	120	0.089	0.107	0.414	0.010
Yarrowia lipolytica PO1f-PYPP	18.60 ± 0.69	6.89	120	0.155	0.186	0.562	0.022
Yarrowia lipolytica PO1f-GK	21.09 ± 2.16	4.68	120	0.176	0.211	0.463	0.038
Yarrowia lipolytica PO1f-TKL	11.10 ± 2.76	4.68	120	0.092	0.111	0.471	0.020
Yarrowia lipolytica PO1f-PYP-GK	23.98 ± 0.15	12.74	120	0.200	0.240	0.792	0.016
Yarrowia lipolytica PO1f-PYP-TKL	15.26 ± 2.84	6.89	120	0.127	0.153	0.581	0.018
Yarrowia lipolytica PO1f-PYP-GK-TKL	27.48 ± 0.67	9.23	96	0.286	0.275	1.042	0.031

Qery productivity, Yery erythritol yield, Rgly glycerol consumption rate, DCW dry cell weight, CPery cell production rate

ISSN: 2731-3654