Table 2 Plasmids and strains used in this study
| Â | Description | Reference |
|---|---|---|
Plasmids | ||
 pHR_A08 | UAS1B8-TEF-CYC1, URA, AmpR | This study |
 pINT3 | UAS16B-CYCt, LEU, AmpR | This study |
 pCHPH | TEFintron-CYC1, HYG, AmpR | This study |
 pHR_A08_PYP | UAS1B8-TEF-PYP-CYC1, URA, AmpR | This study |
 pINT3_GK | UAS16B-GK-CYCt, LEU, AmpR | This study |
 pCHPH_TKL | TEFintron-TKL-CYC1, HYG, AmpR | This study |
Strains | ||
 Escherichia coli-10 beta | Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) | New England Biolabs |
 Yarrowia lipolytica PO1f | MATa LEU2-270 URA3-302 xpr2-322 axp1 | ATCC MYA-2613 |
 Yarrowia lipolytica PO1f-A08 | Y. lipolytica PO1f integrated with linearized plasmid pHR_A08, URA +  | This study |
 Yarrowia lipolytica PO1f- pINT3 | Y. lipolytica PO1f integrated with linearized plasmid pINT3, LEU +  | This study |
 Yarrowia lipolytica PO1f- pCHPH | Y. lipolytica PO1f integrated with linearized plasmid pCHPH, HYG +  | This study |
 Yarrowia lipolytica PO1f-A08-pINT3 | Y. lipolytica PO1f-A08 integrated with linearized plasmid pINT3, URA + , LEU +  | This study |
 Yarrowia lipolytica PO1f-A08-pCHPH | Y. lipolytica PO1f-A08 integrated with linearized plasmid pCHPH, URA + , HYG +  | This study |
 Yarrowia lipolytica PO1f-A08-pINT3-pCHPH | Y. lipolytica PO1f-A08-pINT3 integrated with linearized plasmid pCHPH, URA + , LEU + , HYG +  | This study |
 Yarrowia lipolytica PO1f-PYP | Y. lipolytica PO1f integrated with linearized plasmid pHR_A08_PYP, URA +  | This study |
 Yarrowia lipolytica PO1f-GK | Y. lipolytica PO1f A08 integrated with linearized plasmid pINT3_GK, LEU +  | This study |
 Yarrowia lipolytica PO1f-TKL | Y. lipolytica PO1f pINT3 integrated with linearized plasmid pCHPH_TKL, HYG +  | This study |
 Yarrowia lipolytica PO1f-PYP-GK | Y. lipolytica PO1f-PYP integrated with linearized plasmid pINT3_GK, URA + , LEU +  | This study |
 Yarrowia lipolytica PO1f-PYP-TKL | Y. lipolytica PO1f-PYP integrated with linearized plasmid pCHPH_TKL, URA + , HYG +  | This study |
 Yarrowia lipolytica PO1f-PYP-GK-TKL | Y. lipolytica PO1f-PYP-GK integrated with linearized plasmid pCHPH_TKL, URA + , LEU + , HYG +  | This study |