Skip to main content

Table 2 Plasmids and strains used in this study

From: Metabolic engineering of the oleaginous yeast Yarrowia lipolytica PO1f for production of erythritol from glycerol

  Description Reference
Plasmids
 pHR_A08 UAS1B8-TEF-CYC1, URA, AmpR This study
 pINT3 UAS16B-CYCt, LEU, AmpR This study
 pCHPH TEFintron-CYC1, HYG, AmpR This study
 pHR_A08_PYP UAS1B8-TEF-PYP-CYC1, URA, AmpR This study
 pINT3_GK UAS16B-GK-CYCt, LEU, AmpR This study
 pCHPH_TKL TEFintron-TKL-CYC1, HYG, AmpR This study
Strains
 Escherichia coli-10 beta Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) New England Biolabs
 Yarrowia lipolytica PO1f MATa LEU2-270 URA3-302 xpr2-322 axp1 ATCC MYA-2613
 Yarrowia lipolytica PO1f-A08 Y. lipolytica PO1f integrated with linearized plasmid pHR_A08, URA +  This study
 Yarrowia lipolytica PO1f- pINT3 Y. lipolytica PO1f integrated with linearized plasmid pINT3, LEU +  This study
 Yarrowia lipolytica PO1f- pCHPH Y. lipolytica PO1f integrated with linearized plasmid pCHPH, HYG +  This study
 Yarrowia lipolytica PO1f-A08-pINT3 Y. lipolytica PO1f-A08 integrated with linearized plasmid pINT3, URA + , LEU +  This study
 Yarrowia lipolytica PO1f-A08-pCHPH Y. lipolytica PO1f-A08 integrated with linearized plasmid pCHPH, URA + , HYG +  This study
 Yarrowia lipolytica PO1f-A08-pINT3-pCHPH Y. lipolytica PO1f-A08-pINT3 integrated with linearized plasmid pCHPH, URA + , LEU + , HYG +  This study
 Yarrowia lipolytica PO1f-PYP Y. lipolytica PO1f integrated with linearized plasmid pHR_A08_PYP, URA +  This study
 Yarrowia lipolytica PO1f-GK Y. lipolytica PO1f A08 integrated with linearized plasmid pINT3_GK, LEU +  This study
 Yarrowia lipolytica PO1f-TKL Y. lipolytica PO1f pINT3 integrated with linearized plasmid pCHPH_TKL, HYG +  This study
 Yarrowia lipolytica PO1f-PYP-GK Y. lipolytica PO1f-PYP integrated with linearized plasmid pINT3_GK, URA + , LEU +  This study
 Yarrowia lipolytica PO1f-PYP-TKL Y. lipolytica PO1f-PYP integrated with linearized plasmid pCHPH_TKL, URA + , HYG +  This study
 Yarrowia lipolytica PO1f-PYP-GK-TKL Y. lipolytica PO1f-PYP-GK integrated with linearized plasmid pCHPH_TKL, URA + , LEU + , HYG +  This study