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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: The phenomenon of degeneration of industrial Trichoderma reesei strains

Fig. 1

Occurrence of a (cel −) population during cellulase production. T. reesei Iogen-M10 strain was cultivated in a 14-L bioreactor under cellulase producing conditions. a Samples were taken every 24 h and used for determination of biomass formation (g/L; blue triangles), protein content (g/L; red diamonds), specific productivity (mg/g/h; green squares) and cellulase activity [% (cel −) population; yellow dots]. b For determination of cellulase activity the samples were spread on PDA plates for sporulation and then transferred to plates containing 1% (w/v) ASC and incubated for 144 h. The number of colonies without a halo, i.e., (cel −) colonies, and the number of colonies with a halo, i.e., (cel  +) colonies were counted. c Determination of the development of a (cel −) population in samples after 72 and 96 h of cultivation in the presence (blue) and absence of (red) of Trichostatin A. Values are means of three replicates, error bars indicate standard deviations

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