Fig. 4

Analysis of the chromatin status upstream of the cbh2 gene and its transcript formation. a Schematic drawing of the upstream region of the cbh2 gene. The black bars indicate the regions chosen for the CHART-PCR. Triangles indicate putative binding sites for transcription factors (colour-code provided). TATA indicates the position of a TATA-like box. Numbers on top indicate distance from ATG. T. reesei Iogen-M10 (blue bars) and Iogen-M10 (cel −) (orange bars) strains were pre-grown and transferred to sophorose (SO) or medium without carbon source (NC) and incubated for 3 h. b CHART-PCR analysis of the two chosen regions. The chromatin compaction index (CCI) indicates the accessibility of DNA to DNase I digestion using normalization to sar1 and act upstream regions. Higher CCI values indicate a more compact chromatin structure. c Transcript levels of cbh2. Transcript analysis was performed by RT-qPCR using sar1 and act genes for normalization. Results are given as relative transcript ratios in logarithmic scale and refer to a reference sample (QM6a, NC). All values are the means of results from three independent experiments. Error bars indicate standard deviations