Fig. 8

Determination of the degeneration rate after growth in conditioned medium. a Schematic presentation of the experimental workflow. T. reesei Iogen-M10 and Iogen-M10 (cel −) strains were grown on 1% lactose for 48 h. Supernatants were sterile filtered and used to mix with fresh medium in the indicated ratio. T. reesei QM6a, Rut-C30 and Iogen-M4 strains were submitted to the ISD protocol under standard condition and using the mixed medium. b The degeneration rate was determined and is given in % of the whole population. The three bars represent the biological triplicates. If no bar is depicted, no (cel −) colonies were observed