Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries

Table 1 Kinetic parameters and specific activity of xylanases against beechwood xylan at 40 °C

	K_m (mg/mL)	k_cat (s⁻¹)	V_max (μmol/min·mg)	k_cat/K_m (mL/s·mg)	Specific activity (U/mg)
XYL10C_∆N	0.96 ± 0.18	530 ± 40	830 ± 62	550 ± 60	710 ± 45
M53S/F54L	1.30 ± 0.10	1090 ± 44	2140 ± 69	1050 ± 31	1990 ± 77
N207G	0.85 ± 0.01	1110 ± 6	1750 ± 9	1300 ± 11	1370 ± 64
M53S/F54L/N207G	0.83 ± 0.10	1260 ± 44	1980 ± 70	1530 ± 144	2090 ± 197

ISSN: 2731-3654