Fig. 3From: Tryptophan plays an important role in yeast’s tolerance to isobutanolGrowth assay related to the pentose phosphate pathway and isobutanol tolerance. a Growth assay was applied to two pentose phosphate pathway (PPP) defective strains (Sc1 and Sc3). The two mutant strains showed more sensitive to isobutanol (1%) compared to the wild type. b, c Two PPP-defective strains (Sc1 and Sc3) and two tryptophan pathway defective strains (Sc7 and Sc12) were used in recovery assays and the two tryptophan pathway defective strains were recovered earlier (b) than the two PPP-defective strains (c). d All defective strains were recovered at 70 h. The recovery assays was performed at 200 μg/ml tryptophan additionBack to article page