Table 1 Biotechnological production of LNT II through enzymatic and cell factory approaches

Single-enzyme

pNP-NAG, β-lactose

Purified β-N-acetylhexosaminidase

70 °C, pH 5.0

4.7 g L⁻¹

[14]

OPME

GlcNAc, β-lactose

Purified Nahk, GlmU, PpA, LgtA

30 °C, pH 8.0

1.54 g (70 mL reaction mixture)

[9]

OPME

Chitin, β-lactose

PbChi70, HaHex74

40 °C, pH 7.5

8.6 g L⁻¹

[8]

E. coli (ΔlacZ)

Glycerol, β-lactose

Chromosomally integrated LacY and LgtA

30 °C, 90 rpm, shake flask (250 mL)

2.465 g L⁻¹

[15]

E. coli (ΔlacZΔnagBΔwecB)

Glycerol, β-lactose

Overexpressed LgtA, GlmM, GlmU, GlmS

25 °C, fed-batch (5-L)

46.2 g L⁻¹

[16]

E. coli (ΔlacZΔnanE)

GlcNAc, β-lactose

Overexpressed NagA, GlmM, GlmU, LgtA

25 °C, fed-batch (3-L)

15.8 g L⁻¹

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