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Table 1 The kinetic parameters of TlGa15B and mutants on soluble starch at 55 ℃

From: Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leycettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications

Enzyme Specific activity (U/mg) Km (mg/mL) Vmax (μmol/min/mg) kcat/Km (mL/s/mg)
TlGa15B 496.2 ± 1.3 0.77 ± 0.08 719.2 ± 19.1 982.3
TlGa15B-GA1 805.8 ± 5.7 0.62 ± 0.07 900.9 ± 23.2 1529.0
TlGa15B-GA2 1054.5 ± 2.2 0.29 ± 0.02 1093.0 ± 22.0 3982.6