Table 1 The kinetic parameters of TlGa15B and mutants on soluble starch at 55 ℃
Enzyme | Specific activity (U/mg) | Km (mg/mL) | Vmax (μmol/min/mg) | kcat/Km (mL/s/mg) |
|---|---|---|---|---|
TlGa15B | 496.2 ± 1.3 | 0.77 ± 0.08 | 719.2 ± 19.1 | 982.3 |
TlGa15B-GA1 | 805.8 ± 5.7 | 0.62 ± 0.07 | 900.9 ± 23.2 | 1529.0 |
TlGa15B-GA2 | 1054.5 ± 2.2 | 0.29 ± 0.02 | 1093.0 ± 22.0 | 3982.6 |