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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis

Fig. 3

Statistical analysis of the MMEJ deletions in the transformants carrying eight self-interference plasmids. a Weblogo analysis of the base preference of the microhomology repeats from all unique MMEJ-mediated deletions. The orders of the bases in the microhomology repeats are indicated. The length of the microhomologies used in each weblogo analysis was ≥ n (n = 1, 2, 3, 4, 5 or 6). b Distribution of microhomology lengths (bp) from all unique MMEJ-mediated deletions. The microhomology lengths is preferred to 0, 6 or 12 bp, marked in red. c GC content of the microhomologous sequences from all unique MMEJ-mediated deletions, and it is preferred to 41–50%, marked in red. d Conservation of junction bases at the adjacent ends of all unique MMEJ-mediated deletions with the microhomologous repeats (n ≥ 1). The 5′- and 3′-adjacnet ends of the deletion region are boxed. The ends are preferred to 5′-A/G-3′, 5′-T/G-3′ and 5′-G/T-3′, marked in red. e The deletion sizes of all unique MMEJ-mediated deletions with the microhomologous repeats (N ≥ 1). The sizes are preferred to 41–50 bp, 61–70 bp, 301–500 bp, marked in red. f Distances between the microhomology repeats (N ≥ 1) and the protospacers. The 5′ upstream (minus) and 3′ downstream (plus) locations of the deletion ends are relative to the 5′-end and 3′ end of the protospacer, respectively

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