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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Fig. 2

Mutations into the pks gene in H. insolens as introduced by CRISPR/Cas9-mediated genome editing. A Disruption of pks was correlated to an albino conidia phenotype in H. insolens. The pks gene disrupted strains displayed pigment-less conidia and resultant white colonies as indicated by red arrows. B Sequence alignment of the pks gene in WT and the pks-edited mutants. The 20-nt protospacer sequence of pks in wild-type strain is represented by blue letters and PAM sequence is labeled by red letters with underline

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