Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Table 2 Parameters calibrated to experimental data of C. owensensis CO80 batch cultures in comparison to the benchmark parameter values of C. saccharolyticus cultures from Ljunggren et al. [21]

Parameter	Benchmark values Ljunggren et al. [21]	10 g/L	30 g/L^a	30 g/L^b	80 g/L
µ_max (h⁻¹)	0.28	0.33 ± 0	0.31 ± 0.082	0.31 ± 0.082	0.29 ± 0.02
K_S (mol/L)	4.8 × 10^–5	4.8 × 10^–3	9.8 × 10^–2 ± 1.5 × 10^–4	4.8 × 10^–5e	0.49 ± 0.064
OSM_crit (mol/L)	0.28	0.23 ± 0.0002	0.39 ± 0.002	0.39 ± 0.002	0.78 ± 0.024
r_cd (h⁻¹)	0.014	0.031 ± 0.0001	0.031 ± 0.0065	0.020 ± 0.00015	0.031^c
\(Y_{{S,{\text{H}}_{2} }}\) (mol/mol)	4.77	3.5 ± 0.38	3.5 ± 0.12	3.5 ± 0.12	2.56^c
Y_S,X (cmol/mol)	4.78	0.79^d	0.80^d	0.80^d	0.72^c
\(n_{{{\text{H}}_{2} }}\)	4.5	5.37 ± 0.00005	5.37^c	5.37^c	4.5^e
n_µ	4.68	4.68^e	4.68^e	4.68^e	4.68^e

Confidence interval 95% is given for those parameters which have been fitted numerically
^aFirst model for the 30 g/L cultures
^bSecond model for the 30 g/L cultures
^cGraphically calibrated
^dCalculated from experimental data
^eSame value as in Ljunggren et al. [21]

ISSN: 2731-3654